Pgps3 Unique Restriction Sites Site

Just remember: no SalI, no EcoRV, and always verify your map. When in doubt, a quick virtual digest takes 30 seconds and saves three days of failed ligations.

If you received PGPS3 from a collaborator or bought a modified version (e.g., PGPS3-EGFP), do this:

For precise mapping, you can refer to the official pGPS3 Sequence Map provided by . pGPS3 - 4293 bp - NEB pgps3 unique restriction sites

: For directional cloning, researchers often use pairs like BamHI and NotI , which are closely spaced (733 and 741 bp) but have distinct overhangs.

PGPS3 is particularly beloved because its backbone lacks many common restriction sites found in standard multiple cloning sites (MCS) of larger vectors—but it also has some notable absences. Just remember: no SalI, no EcoRV, and always verify your map

: Use unique sites to insert your gene of interest into the Transprimer for random delivery into a target plasmid or genome. Selection : The vector carries ampicillin resistance ( ApRcap A p to the cap R-th power ) in its backbone and kanamycin resistance ( KnRcap K n to the cap R-th power

Based on the annotated sequence of PGPS3 (GenBank: FJ876861.1, or similar pGreen-based derivatives), here are the confirmed restriction enzymes. Note: Always verify with your specific plasmid map, as some variants (e.g., PGPS3-FLAG, PGPS3-GFP) may have inserted sites. pGPS3 - 4293 bp - NEB : For

Note: If you are referring to a specific custom plasmid or a different vector (like pGPS3 from New England Biolabs), the map will differ. The data below applies to the standard pGEP3 (GFPS3) sequence, which is roughly 4.7 kb.

A means the recognition sequence appears only once in the entire plasmid backbone. This is gold for molecular cloning because:

Today, we’re going to map out the and discuss how to leverage them for seamless construct assembly.

Helen Plum Library

Pgps3 Unique Restriction Sites Site