750 mM NaCl, 50 mM MOPS (pH 7.0), 15% Isopropanol, 0.15% Triton X-100. 1.0 M NaCl, 50 mM MOPS (pH 7.0), 15% Isopropanol. QF 1.25 M NaCl, 50 mM Tris-HCl (pH 8.5), 15% Isopropanol. Spin Column & DNA Cleanup Buffers Buffer PE (Wash) : 10 mM Tris-HCl (pH 7.5), 80% Ethanol.
and Buffer PB contain NaOH or high guanidine-HCl, which can cause irritation. Use appropriate PPE. qiagen buffer recipes
| Buffer | Hazard | |--------|--------| | P2 (NaOH) | Caustic – eye/skin burn | | PB, AL, RLT | Guanidine – toxic, irritant | | PE (EtOH) | Flammable | | β-ME | Nasty odor, toxic – use fume hood | 750 mM NaCl, 50 mM MOPS (pH 7
The starting line. This buffer resuspends bacterial pellets while inhibiting nuclease activity. Spin Column & DNA Cleanup Buffers Buffer PE
SDS solution. . If SDS precipitates, warm to 37°C before use . Buffer N3 (Neutralization Buffer)
